The histological and immunohistochemical aspects of bile...

GastroenterolRes Pract. 2011;2011:905872. Epub 2011 Jul 24.

Introduction. The pathogenesis of GERD isstrongly related with mixed acid and bile reflux. Benign and malignantesophageal and gastric lesions have been associated with synergetic activity betweenthose parameters. Bile reflux causes reactive gastropathy evaluated with BileReflux Index (BRI). The aim was to investigate if the sequence: bilereflux-intestinal metaplasia-GERD-esophagitis, is associated withapoptotic/oncogenetic disturbances.四川省第四人民医院消化内科常玉英

Materials/Methods. Fifteen asymptomaticsubjects and 53 GERD patients underwent gastroscopy with biopsies. Thespecimens examined histologically and immunohistochemically for p53, Ki-67,Bax, and Bcl-2.

Results. Elevated BRI score detected in 47% (25/53) of patientswith GERD and in 13% (2/15) of controls (P = 0.02). Severe esophageal lesionswere significantly more common in BRI (+) patients (14/25) compared to BRI (-)ones (P = 0.0049). Immunohistochemical analysis did not show associationsbetween BRI score and biomarker expression. Conclusions. Bile refluxgastropathy is associated with GERD severity, but not with oncogene expressionor apoptotic discrepancies of the upper GI mucosa.